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Avances en Medicina Regenerativa aplicada a la Urología.

Publicación conjunta: La revista “Urology” (Impact Factor: 2,43), ha aceptado para su publicación el trabajo:...


Avances en Medicina Regenerativa aplicada a la Urología.

Publicación conjunta: La revista “Urology” (Impact Factor: 2,43), ha aceptado para su publicación el trabajo: “Histological changes post urethroplasty using small intestinal submucosa (SIS) unseeded with cells in rabbits with injured urethra” de los autores: Villoldo Gustavo (Investigador Urología Regenerativa), Loresi Mónica (ICBME), Giúdice Carlos (Urología), Damia Oscar (Urología), DeBadiola Francisco (Cirugía Pediátrica), Moldes Juan (Cirugía Pediátrica), Barbich Mariana (ICBME) y Argibay Pablo (ICBME).

Este trabajo es la primera publicación internacional que la Unidad de Urología Regenerativa produce. Sin embargo, en los últimos dos años ha sido presentado parcialmente en reuniones médicas nacionales e internacionales, ameritando premios en la especialidad.

La Unidad de Urología Regenerativa, se creó como un área transdisciplinaria de investigación en Medicina Regenerativa e Ingeniería de Tejidos, con el objetivo de aplicar células madre y diferentes matrices a la reparación de defectos urológicos en niños y adultos. Dicha Unidad está integrada por los Servicio de Urología y Cirugía Pediátrica, asociados con el área de Medicina Regenerativa del ICBME. Plasma de esta manera una conjunción entre la investigación básica, la cirugía experimental y la urología asistencial.

Abstract (authors sample):
OBJECTIVE To determine whether small intestine submucosa has the same regenerative capacity when urethroplasty is performed in injured urethras.

METHODS Our experiment was conducted in 30 New Zealand male rabbits, all of which had urethral injury. One month after the injury, the animals were randomized into a control group or a group with only urethroplasty with small intestine submucosa. The animals were euthanized at 2, 4, 12, 24, and 36 weeks after urethroplasty, and their urethras were removed for histologic and immunohistochemical examination. Before the scheduled euthanasia, urethrography and cystoscopy were performed.

RESULTS After 2 weeks, there was evidence of a continuous monolayer of stratified epithelial cells and absence of smooth muscle fibers. One month later, the epithelium showed no changes from the previously observed features, but some smooth muscle fibers (representing newly formed vessels), became apparent. After 3 months, the graft showed increased concentration of smooth muscle fibers. After 6 and 9 months, the density of smooth muscle cells remained unchanged. Fiber arrangement was irregular, particularly at the anastomosis site. Epithelial and smooth muscle phenotypes were confirmed by immunohistochemistry using anti-pan-citokeratin (AE1/AE3) antibodies and anti-a-smooth muscle actin, respectively.

CONCLUSION Small intestine submucosa promotes regeneration in traumatized urethras, with slightly delayed. epithelialization and abnormal distribution of smooth muscle. Urethral damage caused by trauma nterferes with the normal healing process. UROLOGY 2013 Elsevier Inc.